The commissioning testing of methodical recommendations for RNA viruses’ identification using next-generation sequencing (pyrosequencing) were successfully completed in the Reference Laboratory for Avian Viral Diseases of the FGBI “Federal Centre for Animal Health” subordinate to the Rosselkhoznadzor.
The proposed methodical recommendations enable to perform genotyping of well-studied and widely spread viruses as well as of understudied viruses including different satellite viruses. Currently the technique is applied in research and scientific activities aimed at the study of isolates of avian influenza, Newcastle disease and infectious bronchitis viruses.
Under conditions of possible introduction of previously unknown and exotic infections into the RF territory, due to the emergence of recombinant forms evolving as a result of wide and often uncontrolled use of live vaccines in poultry production it is necessary to develop and improve the techniques of pathogen detection and identification. The combination of high throughput sequencing with conventional methods of molecular biology, like polymerase chain reaction (PCR) and Sanger sequencing as well as with the latest computer developments in bioinformatics facilitate intensification of activities aimed at the study of infectious agent genetic determinants.
The Center plans to use the developed methodical recommendations actively in several scientific studies associated with the infectious agent genetic analysis.
FGBI “ARRIAH” Press Office