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Range of drugs for animals is constantly increasing which makes the choice of the most suitable drug quite difficult. Nevertheless, considering the fact that conventional antibacterial drugs do not always meet current requirements (due to insufficient efficacy) monitoring of the structure and level of microorganism resistance to drugs has become quite important. Subordinate to the Rosselkhoznadzor FGBI Federal Centre for Animal Health performs investigations aimed at determination of antibiotic resistance of isolates of bacterial agents of porcine respiratory diseases recovered in the RF farms. This information is essential for development and application of scientific approaches to treatment of porcine respiratory diseases, prevention of emergence and spread of antibiotic resistant microorganisms in pig farms.

149 isolates of porcine respiratory disease agents were tested since 2012 till 2014: Pasteurella multocida – 38, Actinobacillus pleuropneumoniae – 42, Haemophillus parasuis – 20, and Streptococcus suis – 49.

Isolate sensitivity to antibacterial drugs was tested using disk diffusion test according to the recommendations of Clinical and Laboratory Standards Institute, USA. Discs pre-impregnated with 22 antimicrobial drugs were tested: penicillin, ampicillin, amoxicillin, amoxicillin-clavulanic acid, ceftriaxone, cefepime, cefotaxime, enrofloxacin, ciprofloxacin, flumecvin, erythromycin, tylosin, spiramycin, tetracycline, chlortetracycline, oxytetracycline, doxycycline, cotrimoxazole, trimethoprim, tiamulin, colistin, and florfenicol.

Most of P. multocida isolates demonstrated high sensitivity to penicillin (76%), aminopenicillins (≥87%), cephalosporin of III-IV generation (100%), fluroquinolone (≥90%), macrolids (≥87%), colistin (92%) and florfenicol (100%). Only 43% pasterella isolates were highly sensitive and 29% - resistant to gentamicin. Macrolides showed good results. The situation on using tetracyclines, cotrimoxazole and trimethoprim, raises concern of the FGBI ARRIAH scientists. More than 30% of all tested pasterella isolates turned out to be resistant to these drugs. Antibiotic of Pleuromutilin group (tiamulin) was effective against 75% P. multocida isolates.

Most of A. pleuropneumoniae isolates were sensitive to penicillin (86%), aminopenicillins (93%), aminopenicillins of III-IV generation (100%), fluroquinolones (except for flumecvin ) (≥87%), and macrolides (≥72%).

No A. pleuropneumoniae isolates totally resistant gentamicin were detected, though 78% isolates demonstrated moderate resistance. Actinobacilli demonstrated resistance to tetrocyclines, cotrimoxazole and trimethoprim more often than pasterella. Besides, there were detected 17% A. pleuropneumoniae isolates resistant to colistin and 5% to florfenicol.

Most isolates sensitive to antibacterial drugs among Pasteurellaceae pathogens were detected in the agent of porcine polyorrhymenitis. Number of H. parasuis isolates resistant to penicillin and aminopenicillines did not exceed 5%. The agent demonstrated good results in sensitivity to the rest of the tested antibacterial drugs.

Most isolates of porcine streptococcus agent were sensitive only to β-lactam antibiotics. S. suis sensitivity to flumecvin and colistin was not tested due to natural resistance of the agent to these drugs. Number of isolates resistant to the rest of antibacterial drugs was greater than in other agents. Number of streptococcus isolates resistant to gentamicin almost two times exceeded the number of resistant pasterella and hemophilic bacteria. Number of S. suis isolates resistant to macrolides was (≥66%), to tetracyclines (≥76%), cotrimoxazole (36%), trimethoprim (44%), florfenicol (16%), and tiamulin (42%).

Comparatively high level of resistance to gentamicin, tetracyclines, sulfanilamides and trimethoprim (more than 30%) limits possibilities of its usage for effective treatment of porcine respiratory diseases of bacterial etiology.

Despite extensive use of antibacterial drugs for treating respiratory pathology on pig farms efficacy of antibiotic treatment varies considerably. Once again detection of sensitivity of recovered agent isolates to antibiotics in each specific farm proves to be essential.

FGBI “ARRIAH” Press Office

FGBI “ARRIAH” Press Office

Источник: http://www.arriah.ru/en/main/news/recertification-current-quality-system-fgbi-arriah-compliance-requirements-gost-p-52249-20

FGBI “ARRIAH” Press Office

Источник: http://www.arriah.ru/en/main/news/recertification-current-quality-system-fgbi-arriah-compliance-requirements-gost-p-52249-20

FGBI “ARRIAH” Press Office

Источник: http://www.arriah.ru/en/main/news/recertification-current-quality-system-fgbi-arriah-compliance-requirements-gost-p-52249-20

Источник: http://www.arriah.ru/en/main/news/avian-influenza-epidemic-situation-worldwide-and-rf

FGBI “ARRIAH” Press Office